Spotlight: Fmoc-Asp(OtBu)-Wang Resin (100–200 Mesh) – A Backbone for Solid-Phase Peptide Synthesis
In the world of peptide synthesis, success often hinges on the tiny details—like the choice of resin. One resin that continues to prove indispensable in Fmoc-based solid-phase peptide synthesis (SPPS) is Fmoc-Asp(OtBu)-Wang Resin. Whether you’re building a short bioactive peptide or a more complex sequence, this resin helps set a solid foundation—literally.
What Is It?
Fmoc-Asp(OtBu)-Wang Resin is a preloaded polystyrene resin functionalized with Fmoc-protected aspartic acid, where the side chain is protected with a tert-butyl (OtBu) ester. It’s built on a Wang linker, a classic acid-labile linker that allows for cleavage under TFA, yielding peptides with a free C-terminal carboxylic acid.
The 100–200 mesh refers to the particle size, typically around 75–150 µm, balancing high surface area with manageable flow and swelling properties during synthesis.
Why Use This Resin?
Ready to Use: Preloaded with Asp(OtBu), ideal for sequences starting with C-terminal aspartic acid.
Fmoc/tBu Compatibility: Perfect for modern SPPS workflows.
Reliable Cleavage: Wang linker enables mild, acid-mediated cleavage with good yields.
Reproducibility: Consistent particle size ensures even reaction kinetics.
Ideal For…
Peptides requiring C-terminal Asp residues
Bioactive peptides, enzyme substrates, or diagnostic tools
Researchers optimizing solid-phase synthesis efficiency
Storage & Handling Tips
Store dry, at 2–8 °C, away from moisture.
Use under an inert atmosphere if storing long term.
Swell in DMF or DCM before use for optimal coupling efficiency.
Final Thought
Whether you’re working in academia or biotech, Fmoc-Asp(OtBu)-Wang Resin is a go-to building block that simplifies synthesis and delivers clean results. Think of it as the unsung hero supporting the beautiful complexity of your final peptide.